Spleen focus forming virus
WebThe amount of the cell-free infectious virus in the supernatant of infected cells was quantified using a focus-forming unit assay, as previously described . Mouse anti-Flavivirus group antigen antibody, clone D1-4G2-4-15 (Millipore) was used as primary antibody and donkey anti-mouse IgG antibody conjugated with horseradish peroxidase (Jackson … Web1 Apr 1980 · Abstract. Spleen cells removed from BALB/c mice as soon as 48 hr after infection with Friend polycythemia virus were separated by velocity sedimentation at unit gravity. Cell fractions were assayed for the production of spleen focus-forming virus and for Friend murine leukemia virus. The production of both viruses per nucleated cell was up to …
Spleen focus forming virus
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Web1 Sep 1983 · A new isolate of a murine erythroblastosis-inducing spleen focus-forming virus (Cas SFFV), derived from the wild mouse ecotropic murine leukemia virus Cas-Br-M, was further characterized after the ... WebSpleen Focus-Forming Viruses Strains of MURINE LEUKEMIA VIRUS that are replication-defective and rapidly transforming. The envelope gene plays an essential role in initiating …
WebSpleen focus-forming viruses are generated by recombination with endogenous retroviral sequences. Strains of MURINE LEUKEMIA VIRUS that are replication-defective and rapidly transforming. The envelope gene plays an essential role in initiating erythroleukemia (LEUKEMIA, ERYTHROBLASTIC, ACUTE), manifested by splenic foci, SPLENOMEGALY, … WebSpleen Focus Forming Virus. The SFFV envelope gene encodes a membrane glycoprotein, gp55. From: Encyclopedia of Immunology (Second Edition), 1998. Related terms: Gene Therapy; Patient; Transcription Factor; Long Terminal Repeat; Chronic Granulomatous …
WebThe spleen focus-forming virus (SFFV) envelope gene, when introduced into mice in the absence of other SFFV genes, induces acute erythroleukemia Authors L Wolff 1 , S … Web28 Mar 2024 · ACKNOWLEDGMENTS. We are grateful to Dario Vignali for pUHD/TetVP16Puro, Gabriel Nuñez for the spleen focus-forming virus Bcl-x L plasmid, John Cleveland for a c-mycprobe, Charles Sherr for a cyclin D 2 probe, Stephen Adam for monoclonal antibody MAb318, and Maarten Fornerod for affinity-purified hCRM1 …
WebThe Friend spleen focus-forming provirus is 6,296 base pairs (bp) in length. Compared to Moloney murine leukemia virus, it has undergone five major deletions, three substitutions, and a number of minor alterations. Otherwise, these viruses are about 90% homologous.
Web1 Nov 1985 · The polycythemia-inducing strain of Friend virus (FV-P) causes a multistage erythroleukemia in susceptible mice. FV-P is a complex of two viruses, a replication-competent virus [Friend murine... how far is saint pete from sarasotaWeb6 Jul 2004 · To enhance the level of gene expression in hematopoietic cells, we also generated a series of lentiviral vectors incorporating the spleen focus forming virus (SFFV) long terminal repeat (LTR) sequences, and the Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE). how far is saint petersburg from meWebUsutu virus (USUV) is an emerging mosquito-borne arbovirus within the genus Flavivirus, family Flaviviridae. Similar to the closely related West Nile virus (WNV), USUV infections are capable of causing mass mortality in wild and captive birds, especially blackbirds. In the last few years, a massive spread of USUV was present in the avian population of Germany and … how far is salamanca ny to erie paWebLentivirus vectors encoding the proinsulin gene (with or without a Kozak sequence) under the control of spleen focus forming virus, cytomegalovirus, elongation factor 1α and … how far is salado tx from dallas txWebA series of insertion-deletion mutants was constructed in a molecularly cloned DNA copy of the Friend strain of spleen focus-forming virus (SFFV). The mutants were produced by inserting a synthetic oligonucleotide linker containing the recognition sequence of SalI endonuclease into several different locations of the SFFV DNA. how far is saipan from iwo jimaWebThe spleen focus-forming virus (SFFV), a replication-defective murine leukemia virus that causes the rapid transformation of certain hematopoietic target cells, has acquired … high caliber wichita falls texasWeb20 Aug 2016 · Replication-competent retroviruses such as Moloney murine leukemia virus (MoMLV) or mouse mammary tumor virus (MMTV) have been used in insertional mutagenesis screens. ... For example, a self-inactivating LV vector with a strong internal spleen focus forming virus (SFFV) promoter was capable of dysregulating nearby genes … high caliber work