Iptg cas
WebIsopropyl-beta-D-thiogalactopyranoside C9H18O5S - PubChem Apologies, we are having some trouble retrieving data from our servers... PUGVIEW FETCH ERROR: 403 Forbidden … WebDec 20, 2024 · The greatest Cas9 protein expression in defined media containing glucose was observed at an induction temperature of 30 °C and with 8 h of post induction time …
Iptg cas
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Web420291 Millipore IPTG, Animal-Free, High Purity - CAS 367-93-1 - Calbiochem Download Zoom Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular … WebIPTG也可用于重组蛋白的诱导表达。在这些体系中，待研究蛋白在IPTG诱导型启动子下游编码。IPTG诱导待研究蛋白表达，对细胞裂解后，使用一系列合适方法来纯化蛋白，如His-tag或GST-tag纯化系统（融合相应标签的重组蛋白）。 IPTG产品特性. 1）CAS NO：367-93-1
Web420291 Millipore IPTG, Animal-Free, High Purity - CAS 367-93-1 - Calbiochem Download Zoom Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no … WebSubstance name : IPTG (Isopropyl β-D-1-thiogalactopyranoside) CAS No : 367-93-1 Product code : R036 Formula : C9H18O5S 1.2. Relevant identified uses of the substance or mixture and uses advised against Use of the substance/mixture : Laboratory chemical 1.3. Details of the supplier of the safety data sheet
WebApr 12, 2024 · Using an IPTG-inducible promoter, we further showed that the CreT RNA with an efficient start codon (AUG or GUG) markedly impaired the growth of AYE cells in medium containing 0.2 mM IPTG (with ... WebDescription; Overview: The lac operon inducer IPTG (isopropyl-β-D- 1-thiogalactopyranoside) is recommended for blue/white screening by lacZ α- complementation with appropriate vectors and host strains, such as pSTBlue-1/NovaBlue, and for inducing protein expression with the pET System and other lac promoter-derived expression systems. The preparation …
WebBiological Activity for IPTG. IPTG is an used in combination with X-GAL (Cat. No. 5510) in cloning procedures requiring expression of β -galactosidase activity. Non-metabolizable analog of galactose. This product has <0.1% dioxane content.
WebCAS Number: 367-93-1 IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant ... ef125 flail mowerWeb当DNA片段插入至pUC系列载体（或其他带有lacZ、Amp基因载体），然后转化至lacZ缺失细胞中后，涂布上述的IPTG、X-gal、Amp平板培养基，可根据长出菌体的蓝白色，而方便地挑选出基因重组体（白色为具有DNA插入片段的基因重组体）。. 蓝白斑筛选的原理及方法. 载体 … ef100mm f2.8lマクロ is usmWebApr 13, 2024 · IPTG (CAS 367-93-1) Glentham Life Sciences GC6586 IPTG Synonyms: Isopropyl β-D-thiogalactoside; Isopropyl β-D-1-thiogalactopyranoside CAS: 367-93-1 MF: C9 H18 O5 S MW: 238.30 Assay (HPLC): 98.0 - 101.0 % Availability: In stock SDS Specification Code Packaging Stock ℹ Dispatches Size No. Units Price (USD) GC6586-5G … ef 100 400mm f4 5 5 6l is ii usm profotWebCAS Number: 367-93-1. Chemical Formula: C 9 H 18 O 5 S. Molecular Weight: 238.3 g/mol. IPTG, dioxane free is an analog of lactose that inactivates the lac repressor resulting in IPTG induction. The lac repressor is a DNA binding protein that inhibits gene expression therefore you can think of IPTG operating by inhibiting an inhibitor thus ... ef12 bluetoothWebIPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. ... IPTG Chemical Structure. CAS No. : 367-93-1. Size Price Stock Quantity; Free Sample (0.1 - 0.5 mg) Apply Now : Solution ... ef15ar01 bnccWebCAS No 367-93-1 Synonyms IPTG; Isopropyl-beta-D-thiogalactoside (Dioxane-free) Recommended Use Laboratory chemicals. Uses advised against Food, drug, pesticide or biocidal product use. Details of the supplier of the safety data sheet Emergency Telephone Number CHEMTRECÒ, Inside the USA: 800-424-9300 CHEMTRECÒ, Outside the USA: 001 … ef 100-400mm f/4 5-5 6l is ii usmWebApr 7, 2024 · 英文品名：IPTG. 货号：1122. CAS ... IPTG可诱导载体lac操纵子DNA区段合成β-半乳糖苷酶氨基端片段，该片段可与宿主细胞编码的缺陷型β-半乳糖苷酶实现基因内互补(α互补)。实现α互补的细菌暴露IPTG，铺在含有X-gal生色底物的培养基上，可形成蓝色菌落 … ef100t199 bradford white