WebTwo cDNA were chosen and cloned into yeast-expressing vector [ pYES2-NaCl-158 and pYES2-NaCl-167 ], and transferred into yeast strain INVSc1 for salt-tolerant test. The … Web1 apr. 2024 · In yeast S. cerevisiae, squalene is synthesized as the first precursor via the mevalonate pathway to produce sterols, such as ergosterol (Fig. 1) []. 3-Hydroxy-3 …
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Web24 apr. 2024 · S. cerevisiae INVSc1 and YPH499 (Invitrogen, USA) were used as the parent strains for the engineered yeast strains. The engineered yeast strains were cultivated at … Web4 jan. 2024 · Yeast cells were transformed using the standard lithium acetate transformation protocol (Gietz et al., 1995). Approximately 200 ng of plasmid p414-TEF 1p-Cas9-CYC1t (DiCarlo et al., 2013) was used to transform yeast strain InvSc1 [MATa his3Δ1 leu2 trp1-289 ura3-52 MATα his3Δ1 leu2 trp1-289 ura3-52] to obtain flowers greer sc
Frontiers Characterization of a Pentacyclic Triterpene ...
WebThe recombinant yeast and INVSc1 were inoculated into 10 mL liquid YPD medium with PDCS as the sole carbon source and static cultured for fermentation. The samples were … Web31 jul. 2024 · The pYES2-GsPRX9-transformed INVSc1 yeast grew better than the pYES2 empty-vector-transformed INVSc1 yeast on a YPD medium containing 0.5 or 0.8 M NaCl (Figure 3 A). In addition, when the salt-sensitive yeast mutant G19 was used, pYES2- GsPRX9 -transformed yeast showed better growth than the pYES2 empty-vector … WebSaccharomyces Cerevisiae Strain Invsc1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … flowers grandville michigan